Cardioprotective Effects in Ethanolic Extract of Grewia Umbelliferea on Isoproterenol-Induced Myocardial Infarction in Albino Rats

The present study was designed to investigate whether Grewia umbelliferea, a natural herb, would attenuate the acute myocardial
infarction in isoproterenol (ISP)-treated rat model via maintaining cardiac function and activities of endogenous antioxidant enzymes.
Heart tissue enzyme analysis in albino (Wistar) male rats, such as LPO, GSH, GPX, GST, SOD, CAT, CK-MB, MDA and biochemical
analysis in serum plasma viz., ALT, AST, LDH, CPK were performed. The lethal doses (LD50) of the plant extract was determined by
method of Lorke using 13 rats. In the first phase rats were divided into 3 groups of 3 rats each and were treated with the ethanol extract
of the seed at doses of 10, 100 and 1000 mg/kg body weight. They were observed for 24 h for signs of toxicity. In the second phase 4
rats were divided into 4 groups of 1 rat each and were also treated with the ethanolic extract at doses of 1000, 1600, 2900 and 5000
mg/kg bodyweight. The median lethal dose (LD50) was calculated using the second phase. Ethanolic extract of the plant at the dose of
(100& 200 mg/kg/p.o) produced significant salvages the heart from isoproterenol induced myocardial ischemic injury. The results
indicate that plant administration causes myocardial adaptation by augmenting endogenous antioxidants and protects rat hearts from
decline in cardiac function and oxidative stress associated with the plant induced myocardial injury, and justify its potential therapeutic
value in the treatment of ischemic heart diseases in albino rats.