Comparative Study between Percolation and Ultra sonication for The Extraction of Hibiscus and Jasmine Flowers Utilizing Antibacterial Bioassay

Objective: In order to determine if different extraction techniques can produce extracts of divergent active compounds from same plants.
Method:Percolation with soxhlet and ultrasonication were used with ethanol solvent to extract hibiscus and jasmine flowers. These extracts were tested for antibacterial effect on Escherichia coli and Staphylococcus aureus with susceptibility test. The study was conducted by extracting the flowers with soxhlet and ultrasonic apparatus using ethanol as solvent. The extracts were separated from the solvent by evaporating the solvent with rotary evaporator. Discs of 6 mm were prepared from filter paper and were impregnated with the extracts. Sub-cultured test organisms, E. coli and S. aureuswere swabbed on Mueller Hinton (MH) agars for susceptibility testing. The prepared discs were placed on the swabbed MH agar plates. Commercially prepared jasmine oil and hibiscus tea extracts’ discs were used as standards. Gentamicin discs were used as positive control and ethanol impregnated discs were used as negative control.
Results:Observations were recorded after 24 hours of incubation at 37 ⁰C. Soxhlet and ultrasonic extracts of hibiscus flower showed antibacterial effect on E. coli and S. aureus. The hibiscus standard did not produce any effect on both E. coli and S. aureus. Soxhlet and ultrasonic extracts of jasmine flower showed no effect E. coli. Soxhlet and ultrasonic extracts of jasmine flower showed antibacterial effect on S. aureus. The jasmine standard showed antibacterial effect on both E. coli and S. aureus.