Development and validation of extractive spectrophotometric methods for the estimation of Chlordiazepoxide in tablet dosage forms

Two visible spectrophotometric methods (Method A and B) were developed and validated for the determination of Chlordiazepoxide in bulk and tablet dosage forms. In these spectrophotometric methods, the chromogenic reagents such as Alizarin Red S dye (Method – A) and Aconitic Acid (Method – B) reagents were used for color development for the estimation of Chlordiazepoxide in pure and formulations. The developed methods were validated according ICH guidelines and adopted for the assay of chlordiazepoxide in the bulk drug and formulations.