Development and Validation of Lc Method for the Estimation of Tretinoin in Pharmaceutical Dosageform

A simple, specific, accurate and precise reverse phase high performance liquid chromatographic method was
developed and validated for the estimation of Tretinoin in tablet dosage form. An Inertsil ODS C-18, 5μm column
having 250 x 4.6mm internal diameter in isocratic mode with mobile phase containing acetonitrile: methanol:
0.1% ortho phosphoric acid in the ratio of 75:05:20 (v/v/v) was used. The flow rate was 1.0ml/min and effluents
were monitored at 236nm. The retention time for Tretinoin was 7.005 min. The method was validated for
linearity, accuracy, precision, specificity, limit of detection, limit of quantification and robustness. Limit of
detection and limit of quantification were found to be 0.025ppm and 0.0824ppm respectively and recovery of
Tretinoin from tablet formulation was found to be 99.27%. The proposed method was successfully applied for the
quantitative determination of Tretinoin in tablet formulation