A simple, rapid, accurate, economical UV–Visible First order derivative Spectrophotometric method was developed and validated for simultaneous estimation of Neomycin and Betamethasone in bulk and dosage form. The method involved determination of Neomycin and Betamethasone using first derivative spectrophotometric method at 224nm and 234nm respectively over the concentration range of 2.5 – 15µg/ml and 0.6 – 3.6µg/ml with the Correlation Coefficien(r2) 0.9997 and 0.9991. The recovery studies confirmed accurace of proposed method and low value standardeviation confirmed precession of method. The method is validated as per ICH guidelines.
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