Isolation and Characterization of Antibacterial Substance Produced by Bacilli Isolated from Lonar Lake

By screening for antibacterial substance producing twenty nine bacilli isolated from Lonar lake. In present study all the bacillus species showed that the antimicrobial activity against the E. coli (100%), out of twenty nine, twenty seven (93%) bacillus species showed antimicrobial activity against to K. pneumoniae, seven (24%) were antimicrobial against to P. vulgaris and nine (31%) were found antimicrobial against to E. aerogenes and twelve (41%) Bacillus species showed antimicrobial activity against to S. aureus The antibacterial substance produced by B. asahii, B. gibsonii, B. pseudofirmus, B. pumilus, B. flexus were found heat stable upto 121° C and showed that the antibacterial activity against to E. coli. Antibacterial substance produced by B. flexus, B. lehensis, B. asahii were found heat stable after incubation at 121°C for 10 min and showed antibacterial ac:tivity against to K. pneumoniae. Twenty six bacilli species were active at pH 10-12 and inhibit the growth of E. coli, twenty one (72%) Bacillus species showed antimicrobial activity against to K. pneumoniae, seven (24%) were found antibacterial against S. typhi and eleven (37%) Bacillus species showed antimicrobial activity against to S. aureus. But all antibacterial substance was not showed the activity against to P. vulgaris and E. aerogenes. The antibacterial substance not showed the antibacterial activity against to S. aureus, S. typhi, P. vulgaris, and E. aerogenes at pH 7-9. This study provides primary evidence that haloalkaliphilic bacilli strains are promising sources for antibacterial substance. These bacteria in general represent a new and rich source of secondary metabolites that need to be explored