Abstract
A simple, precise and accurate RP-HPLC method was developed and validated for rapid assay of
Carbidopa in tablet dosage form. Isocratic elution at a flow rate of 1.0ml/min was employed on a
symmetry C18 (250x4.6mm, 5μm in particle size) at ambient temperature. The mobile phase consisted
of methanol: KH2PO4: 75:25 (V/V). The UV detection wavelength was 262 nm and 20μl sample was
injected. The retention time for Carbidopa was 1.48 min. The percentage RSD for precision and
accuracy of the method was found to be less than 2%. The method was validated as per the ICH
guidelines. The method was successfully applied for routine analysis of Carbidopa in tablet dosage
form and bulk drug.
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