new simple, rapid, selective, precise and accurate isocratic reverse phase high performance liquid
chromatography assay has been developed and validated for the estimation of Fosamprenavir in
tablet formulation. The separation was achieved by using C-18 column (250x4.6mm, 5μm in particle
size) at ambient temperature coupled with a guard column of silica in mobile phase Acetonitrile:
Water with the pH value adjusted to 5.1. The flow rate was 1.5ml/min and the drug was detected by
using UV detector at the wavelength 260nm and the run time was 8min. The retention time was
found 3.8 minutes. The percentage of RSD for precision and accuracy of the method was found to be
less than 2%. The method was validated as per ICH guidelines. The proposed method was found to
be accurate, repeatability and consistent. It can be successfully applied for the analysis of the drug in
marketed formulation and could be effectively used for the routine analysis of the same drug
without any alteration in the chromatographic conditions.
Abstract