New Method Development and Validation of Tadalifil Using Uv- Visible Spectrophotometr

Three simple, economical, precise and reproducible visible spectrophotmetric methods have
been developed for the estimation of Tadalifil in bulk formulation. The developed methods are based on
the formation of charge transfer coloured complex of Tadalifil with N-Bromo succinimide, complexes
with chloramine-T and phosphymolybdic acid using double distilled water. The complexes with NBromo
succinimide, chloramine-T and phosphymolybdic acid shows absorbances maxima at 520.0 nm,
540.0 nm 840.0nm and linearity in the concentration range of 1-20 μg/ml, 0.5-1.0 μg/ml and 10-30
μg/ml . Results of analysis for all the three methods were validated statistically and by recovery studies.
The molar absorpitivity, Sandell sensitivity, Corelation coffecient were also calculated.