Abstract
A simple, rapid and precise reverse phase high performance liquid chromatography method was developed
for the analysis of Daptomycin in tablet. Chromatographic separation of Daptomycin was performed by using a
kromosil C18 column (250 x 4.6mm, 5 μm) as stationary phase with a mobile phase comprising of 0.1% Ortho
phosphoric acid : Acetonitrile: Methanol 30:45:25 (v/v) at a flow rate of 1.0ml/min and UV detection at 282nm.
The linearity of Daptomycin is in the range of 0.2 mg/ml to 1.4 mg/ml. The limit of detection for Daptomycin was
found to be 10 nano grams. The recovery was calculated by standard addition method. The proposed method was
found to be accurate, precise and rapid for the analysis of Daptomycin.
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