High resolution RP-HPLC method has been developed for the simultaneous determination of aspirin, Atenolol,
Losartan Potassium and Atorvastatin in pharmaceutical dosage forms. HPLC analysis was carried out by using a
X-Terra RP-18, 150x4.6mm, 5micron column with the gradient mobile phase composed of Sol-A: ammonium
acetate buffer (1.4g of ammonium acetate in to 1000ml of HPLC water) and sol-B: acetonitrile with simple
gradient program (0-4min, sol-A:98-92; 4-8min- sol-A:92-60; 8-13min- sol-A:60-65; 13-15min- sol-A:65-98 and
15-20min- sol-A:98-98). 1.0ml per min flow rate, 40°C column oven temperature and 230nm was selected for this
study. The retention times of Aspirin, Atenolol, Losartan and Atorvastatin were 2.0min, 4.7min, 10.0min and
11.8min, respectively. Percent relative standard deviation for five replicate standard injections area is below
1.5percent. Validated the method with specificity, precision, linearity, accuracy, ruggedness and robustness. The
response was linear over the concentration range of 10 to 60 μg per mL for each ingredient, with correlation
coefficients value is greater than 0.999. Recovery results were between 98.0% to 102.0%. The developed method
has applicable for regular analysis.
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