Issue
Article Subject
Agriculture
Abstract

An efficient regeneration procedure was developed for maize from mature embryos derived from
callus of maize inbred line. We found that 3mg/l 2-4 D in MS medium was optimum for the callus
induction .the induction frequency of primary calli was over 85%. Efficient shoot regeneration was
obtained on regeneration medium containing 0.5mg/l BAP in combination with 0.5 mg/l kinetin.
Regenerated shoots were rooted on half strength MS medium containing 1mg/l IBA. This plant
regeneration protocol provides a foundation for genetic transformation of maize.

Keywords
Maize
MS medium
calli
embryogenesis
Agrobaterium.
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