Abstract
simple, selective, linear, precise and accurate RP-HPLC method was developed and validated for rapid
assay of Stanzolol in tablet dosage form. Isocratic elution at a flow rate of 1.0ml/min was employed on a
symmetry C18 (250x4.6mm, 5μm in particle size) at ambient temperature. The mobile phase consisted
of Methanol: water in the ratio of 90:10 v/v. The UV detection wavelength was 210 nm and 20μl sample
was injected. The retention time for Stanzolol was 4.09min. The percentage RSD for precision and
accuracy of the method was found to be less than 2%. The method was validated as per the ICH
guidelines. The method was successfully applied for routine analysis of Stanzolol in tablet dosage form.
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