A novel, simple and economic reverse phase high performance liquid chromatography (RPHPLC)
method has been developed for the estimation of Paracetamol, nimsulide and tizanidine in bulk
and tablet dosage forms with greater precision and accuracy. Separation was achieved on C18 column
(250X4.6mm , 5μm) using gradient mobile phase Sol-A: buffer (weighed accurately 1gm of ammonium
acetate in to 1000mL of HPLC grade water) and Sol-B: acetonitrile gradient program (0-6min, sol-A:
80-37; 6-10min- sol-A: 37-80 and 10-15min- sol-A: 80-80), pumped in to the column at flow rate of 1
ml/min and the detection of eluent from the column was carried out using variable wavelength
detector at 230nm. The total run time was 15 min and the column was maintained at ambient
temperature. The retention times of paracetamol, tizanidine and nimsulide were 3.0min, 5.3 and
8.6min, respectively. The standard curves were linear over the concentration range of 12.5-75μg/ml
and the % RSD of intraday and inter day precision was found to be good. The method was validated as
per ICH guidelines. Validation studies demonstrated that the proposed RP-HPLC method is simple,
specific, rapid, reliable and reproducible. The high recovery and low relative standard deviation
confirm the suitability of the proposed method for the routine quality control analysis in bulk and
tablet dosage forms.
Abstract
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