Spectrophotometric Determination of Antibiotics in Pharmaceutical Products with Turbidimetry Method by Usingpb4+ and Tangesto Phosphoric Acid

A simple, sensitive,fast and accurate spectrophotometric Turbidimetery method of analysis of antibiotics in pharmaceutical dosage forms has been developed and validated. Unfortunately, usage of these methods in measuring an organic composition, especially in medicines has not been considered. In this method, the necessary and optimum conditions for measuring a antibiotics by using a mixture of reagents deposition of Pb4+ and tangesto phosphoric acid have been reported and to following that, the suggestion method of measuring a antibiotics in pharmaceutical production has been developed. The linear Dynamic range for Tobramycin is 2-23 ppm and for azithromycin is 2.2 -24ppm .Detection limit for Tobramycin is 1.6 ppm and Detection limit for azithromycin is 1.7 ppm. This method is like that antibiotics which is a electron donor cause changing Pb4+ to Pb2+ and Pb2+ is made a sediment with Cl- and create a colloid and every 1 mol of antibiotic is made a mol of Pb2+. In this study,the turbidance of homogenous PbCl2 producted from the above mentioned oxidation-reduction is proportional to concentration of antibiotic,and therefore,we developed an indirect simple,fast,and inexpensive turbidimetric method for the determination of antibiotics in pharmaceutical preparations. By measuring an PbCl2 , the content of existent antibiotics in pharmaceutical production has been calculated.