Issue
2011 volume.1
Article Category
Research Article
Article Subject
Pharmacy
Article Authors
G.Satyavathi
M.Sidhakar
V.Rajesh
K.Satyanarayana
D.Rama Krishna
G.Prathap
J.Suvarchala
Rambabu .Kuchi
Abstract

This paper describes the validation of an isocratic HPLC method for the assay of Efavirenz . This
work is concerned with application of simple, precise, accurate and reproducible reverse phase high performance
liquid chromatographic (RP-HPLC) method for simultaneous estimation of Efavirenz. The method employ a
Zodiac C18 column with Methanol and acetonitrile 80:20 (v/v) as the mobile phase and ultraviolet (UV) detection
at 280 nm. A linear response (r>0.998) was observed over the concentration range of 15-45 μg/ml. The results
showed good recoveries, ranging from 98.77 to 101.45% and the relative standard deviation (R.S.D.) intra-day
and inter-day were ≤ 0.80%.. As the method could effectively separate the drug from its degradation products, it
can be used in stability studies for drug substance.

Keywords
Efavirenz
Method development
validation
280 nm.
Article PDF
PDF (For Download)