Vitro Antimicrobial Activity of Brassica Plant’s Seed

Background: Biological activity is a mirror telling the valuable or unhelpful possessions of crude drug or living substance. For the evaluation of biological activity of natural compound bioassays are performed. In this research antibacterial and antifungal activity of crude ethanolic and ethanolic extract was screened through bioassays.
Objective: The antimicrobial activity of the ethanol and methanol extracts of the plant’s Brassica seed was investigated to verify its medicinal use in the treatment of microbial infections.
Method: The antimicrobial activity of the ethanol extract was tested against clinical isolates of some multidrug-resistant bacteria using the agar well diffusion method while antifungal activity is screened through agar tube dilution method. Commercial antibiotics were used as positive reference standards to determine the sensitivity of the clinical isolates.
Results: Both the ethanolic and methanolic extracts exhibited considerable antibacterial and antifungal activity. Maximum antibacterial activity was shown by methanolic extract of tested plant against Escherichia coli (17 mm), Enterobacter aerogenes (12mm) and Bacillus subtilis (11mm) inhibition zones at the 15 mg/ml concentration while the slandered antibiotics Doxycycline (DOX) showed 18mm inhibition zone. The Minimum inhibitory concentration (MIC) was found 1 mg/ml. The methanolic extract showed a highly significant results inhibition towards the tested fungal microorganisms compared to the ethanolic extract. Maximum antifungal activity was exhibited by the methanolic crude extract against A. fumigates and A. nigar and found to be 85.2% and 79%, respectively.