The present study was undertaken to determine the anti-microbial potential of the methanol extract of different plant parts of Pongamia Pinnata in vivo and in vitro. All the extractions were tested against different gram positive and gram negative bacteria and fungi to find out their antimicrobial activity using agar well diffusion and micro dilution method for fortitude the MIC value. The maximum antimicrobial activity of root extract was observed against E. coli and F. oxisporum while in leaf extract maximum antimicrobial activity was observed against S. aureus, B. cereus and A. solani. However, Callus extracts showed mild to moderate inhibitory effects against E. cloacae, P. aeruginosa, A. flavus and R. stolonifer. Besides these, the maximum antimicrobial activity of stem extract was observed only against A. niger. Flucanazole and Ampicillin standards were used as positive controls respectively for fungi and bacteria in this study.
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