Optimization of Regeneration Protocol for Maize (Zea Mays L) from Callus

Abstract

An efficient regeneration procedure was developed for maize from mature embryos derived from
callus of maize inbred line. We found that 3mg/l 2-4 D in MS medium was optimum for the callus
induction .the induction frequency of primary calli was over 85%. Efficient shoot regeneration was
obtained on regeneration medium containing 0.5mg/l BAP in combination with 0.5 mg/l kinetin.
Regenerated shoots were rooted on half strength MS medium containing 1mg/l IBA. This plant
regeneration protocol provides a foundation for genetic transformation of maize.