Synthesis and pharmacological screening of Pyrazolopyridine compounds as Anxiolytics

Abstract

Amine & chloro derivatives of Ethyl 4-substituted-6-phenyl-4,7-dihydro-1H-pyrazolo[3,4-b]pyridine-5-carboxylate & carboxylic acid were synthesized using liquid ammonia & phosphorous oxychloride from 1-pentyl-5 amino pyrazole respectively. 3 amino-2 substituted-4,7-dihydro-2H-Pyrazolo[4,3-c]pyridine-4,6-diol were synthesized from 3-cyanometyl-4-cyano-5-amino-1-substituted pyrazole using active methylene compound, Malononitrile & substituted hydrazine. Pyrazolo[3,4-b]pyridinone was synthesized from 3-amino-1-phenyl pyrazolone & acetylacetone. Completion of reactions was checked by TLC using hexane:ethyl acetate as mobile phase. Reagents like Diethyl Benzoyl malonate & β-Cyano Ethylhydrazine were also synthesized. All the synthesized compounds were characterized by IR, Mass & NMR analytically & by melting point & TLC physically. The synthesized compounds were tested using Elevated Plus Maze model.