Abstract
A simple, specific, accurate and precise reverse phase high performance liquid chromatographic method was developed and validated for the estimation of Tretinoin in tablet dosage form. An Inertsil ODS C-18, 5μm column having 250 x 4.6mm internal diameter in isocratic mode with mobile phase containing acetonitrile: methanol: 0.1% ortho phosphoric acid in the ratio of 75:05:20 (v/v/v) was used. The flow rate was 1.0ml/min and effluents were monitored at 236nm. The retention time for Tretinoin was 7.005 min. The method was validated for linearity, accuracy, precision, specificity, limit of detection, limit of quantification and robustness. Limit of detection and limit of quantification were found to be 0.025ppm and 0.0824ppm respectively and recovery of Tretinoin from tablet formulation was found to be 99.27%. The proposed method was successfully applied for the quantitative determination of Tretinoin in tablet formulation