Abstract
Sofosbuvir and Ledipasvir are available in solid dosage form to cure hepatitis C. the objective of this study was to develop simple HPLC method for both active components. Preparation of buffer: 1ml of ortho phosphoric acid was diluted to 1000ml with HPLC grade water. Chromatographic conditions are mobile phase: 50% OPA (0.1%): 50% Acetonitrile, flow rate: 1 ml/min, column: Discovery C8 (4.6 x 250mm, 5μm), detector wave length: 230nm, column temperature: 30°C, injection volume: 10L, run time: 7 min, diluent: water and acetonitrile in the ratio 50:50 v/v. method validation was carried out and results confirmed the method ruggedness and stability indicating. Optimized method can be used for regular analysis.
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