Innovative Approaches to the Design and Development of Multiplex Lateral Flow Assays

Abstract:
The lateral flow assay (LFA) is a hasty, simple, and inexpensive qualitative diagnostic tool widely used for the detection of targeted analytes in different specimen types. This test is based on the movement of liquid samples through a porous membrane, enabling enabling binding with labeled antibodies
or other detection reagents.
The basic aspects of LFA are a sample pad, conjugate pad, nitrocellulose membrane, and absorbent pad. If the target analyte is present, it will bind to conjugated specific antibodies that are linked to an easily detectable label, yielding an observable signal along a defined test line embedded in the membrane. This signal is generally localized very near a control line to determine whether or not the test is
accurate.