RP-HPLC Method Development and Validation for the Analyisis of Rifaximin in Pharmaceutical Dosage Forms

Abstract

A simple, selective, linear, precise and accurate RP-HPLC method was developed and validated for rapid assay of RIFAXIMIN in tablet dosage form. Isocratic elution at a flow rate of 1ml min-1 was employed on a symmetry C18 column at ambient temperature. The mobile phase consisted of Acetonitrile: Ammonium Acetate 85:15 (v/v). The UV detection wavelength was at 236nm.Linearity was observed in concentration range of 5-50ppm. The retention time for RIFAXIMIN was 4.3 min. The method was validated as per the ICH guidelines. The proposed method can be successfully applied for the estimation of RIFAXIMIN in pharmaceutical dosage forms.