Tag: 253 nm

Abstract A simple, accurate RP-HPLC method was developed and validated for rapid analysis ofBambuterol in formulation. Isocratic elution at a flow rate of 1.2ml/min was employed on a symmetryChromosil C18 (250x4.6mm, 5μm in particle size) at room temperature. The mobile phase consisted ofMethanol: ACN: TEA 30:67:3 (V/V). The UV detection wavelength was 253 nm and 20μl sample wasinjected. The retention time for Bambuterol was 6.2 min. The percentage RSD for precision and accuracyof the method was found to be less than 2%. The method was validated as per the ICH guidelines. Themethod was successfully applied for regular analysis of Bambuterol in tablet dosage form and bulk drug. [avf_view name="Article Addons" view-id="66d98451c48e3"]