Tag: Aspirin

Abstract High resolution RP-HPLC method has been developed for the simultaneous determination of aspirin, Atenolol,Losartan Potassium and Atorvastatin in pharmaceutical dosage forms. HPLC analysis was carried out by using aX-Terra RP-18, 150x4.6mm, 5micron column with the gradient mobile phase composed of Sol-A: ammoniumacetate buffer (1.4g of ammonium acetate in to 1000ml of HPLC water) and sol-B: acetonitrile with simplegradient program (0-4min, sol-A:98-92; 4-8min- sol-A:92-60; 8-13min- sol-A:60-65; 13-15min- sol-A:65-98 and15-20min- sol-A:98-98). 1.0ml per min flow rate, 40°C column oven temperature and 230nm was selected for thisstudy. The retention times of Aspirin, Atenolol, Losartan and Atorvastatin were 2.0min, 4.7min, 10.0min and11.8min, respectively. Percent relative standard deviation for five replicate standard injections area is below1.5percent. Validated the method with specificity, precision, linearity, accuracy, ruggedness and robustness. Theresponse was linear over the concentration range of 10 to 60 μg per mL for each ingredient, with correlationcoefficients value is greater than 0.999. Recovery results were between 98.0% to 102.0%. The developed methodhas applicable for regular analysis. [avf_view name="Article Addons" view-id="66d98451c48e3"]