Abstract Microbial degradation of chemicals in the environment is an important route for removal of harmful compounds. These compoundsrange from plastics through organic chemicals (both industrial chemicals used in large quantities and trace chemicals such as pesticides)to organometallics such as methylmercury. The largest volume of pesticides produced and sold comprises the chlorinated hydrocarbons.These compounds composed of hydrogen, chlorine and carbon atoms.Microbial degradation of pesticides such as 2, 4,-D, Alachlor, Amitrole, Chlorsulfuron, DDT etc applied to soil is the principlemechanism which prevents the accumulation of these chemicals in the environment. Here in this paper is maily based on identifying thethe organisms that can degrade cholorpyrifos. This is done using techniques mainly by colony counting method. Microorganisms arealso grown on BTB plates which indicate the color change and it’s mainly a PH indicator. Then degradation studies is performed .Fromthis degradation studies the best organism utilizing cholrpyrifos as carbon source is identified and then Thin layer chromatography isperformed. As per the experimental results it is found that SD 31 and CD 31 are the one with good degradation values and utilizes CPPefficiently as the carbon source [avf_view name="Article Addons" view-id="66d98451c48e3"]