Tag: clinical pathogens

Abstract The actinomycetes are existing in various habitats in nature. The terrestrial forms have been extensively used for the productionof secondary metabolites and used to treat infectious diseases. However the extreme counterparts have reminded relativelyunknown and unexploited. The soil from the saltpan regions of Cuddalore, Parangipettai and Portonovo Tamilnadu, India werescreened for the isolation of antagonistic actinomycetes. In the present study, morphologically diverge four actinomyceteisolates were obtained from different sampling station were identified as Streptomyces species. All the four actinomycetes werescreened for preliminary antibacterial activity. Out of four strains screened, one strain Streptomyces sp SBU1 showed mostpromising antagonistic activity against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. Secondaryscreening of the actinomycetes ethyl acetate extract also exhibited optimistic antibacterial activity against P.aeruginosa (22 mm)followed by S.aureus (19 mm) and E.coli (18 mm). Partially purified column chromatography fractions (fraction 1 and 2)showed significant antimicrobial activity against P.aeruginosa (16 mm). A single separate band of the antimicrobial compoundwas observed by TLC. The Rf value of fraction 1 and fraction 2 was 0.83 and 0.75 respectively. The present investigationindicates that the solar saltpan soil harbors diverse species of actinomycetes possessing potent antibacterial activity. [avf_view name="Article Addons" view-id="66d98451c48e3"]