Tag: Enzyme linked Immunosorbent Assay

Abstract Diabetes mellitus is a metabolic disorder that is principally characterized by insulin resistance, relative insulin deficiency and hyperglycemia.The present research work was designed to study the inhibition of glycation with natural Inhibitor “MalusDomistica”. For this purpose, normal plasma was collected from healthy volunteers. To study glycation inhibition, thirty two combinations were made and all these combinations were placed at 37oC at same time for five weeks. Human normal plasma was used as a protein. Four milliliter of samples was drawn after 1st, 2nd, 3rd, 4th and 5th week of incubation to perform the experiments. Browning production was recorded by taking absorbance at 370nm of all combinations. Dialysis was performed to remove the excess/ free glucose. Glucose and protein estimation was done to check the glucose and protein concentration before and after dialysis. Glycation inhibition was measured with TBA assay and also by ELISA. The activity of I2 (10 times diluted) inhibitor was approximately high mainly in the 2nd week of incubation. “MalusDomistica” is effective and efficient in lowering the glycation level in conditions when the level of glucose is high i.e. in diabetes. So the results indicate that in future “MalusDomistica” can be used for lowering glucose level in the body. [avf_view name="Article Addons" view-id="66d98451c48e3"]