Abstract An efficient regeneration procedure was developed for maize from mature embryos derived fromcallus of maize inbred line. We found that 3mg/l 2-4 D in MS medium was optimum for the callusinduction .the induction frequency of primary calli was over 85%. Efficient shoot regeneration wasobtained on regeneration medium containing 0.5mg/l BAP in combination with 0.5 mg/l kinetin.Regenerated shoots were rooted on half strength MS medium containing 1mg/l IBA. This plantregeneration protocol provides a foundation for genetic transformation of maize. [avf_view name="Article Addons" view-id="66d98451c48e3"]