Abstract The numbers of antibiotics ,pain killers are important role in the society particularly animal and humanlife. In this case diclofenac sodium (DFS) is used to treat pain, inflammatory disorders,and dysmenorrheal.Inflamatory disorder may include musculoskeletal complaintsto the animals and human toprevent the different pains. But not only some of the harmful in the nature to the animal and human life. Supposeafter usage of this antibiotic the milk was contaminated with the residues of Diclorofenac sodium. We examine 4different she-buffalos for our research work. In our research work collect the raw milk, boiled milk, and curdmilk and some different food stuff. And also analyzed for Diclofenac sodium (DFS) by using H.P.L.C at 258 nmwith UV detector. In this case satisfactory results were obtained. [avf_view name="Article Addons" view-id="66d98451c48e3"]