Tag: RP-HPLC and dosage forms.

Abstract Novel RP-HPLC method has been developed for simultaneous determination of Meformin HCl,Glipizide and Repaglinide in dosage forms. The separation was achieved on a 3.5-micron C18 column(150 X 4.6 mm) using mobile phase consisting of buffer (1.0gm of Potassium dihydrogen phosphate in1000mL, pH 3.0 with diluted orthophospharic acid .The flow rate was maintained at 1.0 ml/min. Thedetection of the constituents was done using UV detector at 210 nm. The retention time of metformin,glipizide and repaglinide were approximately 1.49, 3.71 and 9.84 min respectively. Recovery studyvalues of three actives were 103% to 99% respectively, relative standard deviation of less than 2%.Linear response obtained for three actives correlation coefficient is not less than 0.999. The proposedmethod was applied for regular analysis and results found to be satisfactory. [avf_view name="Article Addons" view-id="66d98451c48e3"]