Tag: RP-HPLC method

Abstract Reverse phase HPLC method has been developed for the simultaneous quantification of Hydrochlorothiazide,Amlodipine besylate and Nebivolol in pharmaceutical dosage forms. chromatographic test was carried out byusing a Develosil C-8 UG-5, 150x3mm column with the gradient mobile phase composed of Sol-A: ammoniumacetate buffer (1.4g of ammonium acetate in to 1000ml of HPLC water) and sol-B: acetonitrile with simplegradient program (0-4min, sol-A:86-86; 4-8min- sol-A:86-63; 8-13min- sol-A:63-63; 13-15min- sol-A:63-86and 15-20min- sol-A:86-86) with 1.0ml per min flow rate. Column oven temperature maintained at 35°C andUV absorbance measured at 225nm. The retention time of Hydrochlorothiazide is 2.28min, Amlodipinebesylate is 11.24 min and Nebivolol is 12.56min, respectively. Area percent RSD (relative standard deviation)for five replicate standard injections is below 1.5percent. Method validation was performed with specificity,precision, linearity, accuracy, ruggedness and robustness. The response was linear over the concentration rangeof 10 to 60 microgram per mL for each ingredient, with correlation coefficients value is greater than 0.999.Recovery results were satisfactory. The developed method is simple, reproducible and accurate. [avf_view name="Article Addons" view-id="66d98451c48e3"]