Tag: Tretinoin

Abstract A simple, specific, accurate and precise reverse phase high performance liquid chromatographic method wasdeveloped and validated for the estimation of Tretinoin in tablet dosage form. An Inertsil ODS C-18, 5μm columnhaving 250 x 4.6mm internal diameter in isocratic mode with mobile phase containing acetonitrile: methanol:0.1% ortho phosphoric acid in the ratio of 75:05:20 (v/v/v) was used. The flow rate was 1.0ml/min and effluentswere monitored at 236nm. The retention time for Tretinoin was 7.005 min. The method was validated forlinearity, accuracy, precision, specificity, limit of detection, limit of quantification and robustness. Limit ofdetection and limit of quantification were found to be 0.025ppm and 0.0824ppm respectively and recovery ofTretinoin from tablet formulation was found to be 99.27%. The proposed method was successfully applied for thequantitative determination of Tretinoin in tablet formulation [avf_view name="Article Addons" view-id="66d98451c48e3"]