Development and Validation of LC Method for the Analysis of Metoclopramide in Pharmaceutical Dosage form and Plasma

A simple, specific, accurate and precise reverse phase high performance liquid chromatographic
method was developed and validated for the estimation of Metoclopramide in tablet dosage form. An
Zodiac C-18, 5μm column having 250 x 4.6mm internal diameter in isocratic mode with mobile phase
containing Acetonitrile:1%TEA 50:50 (/v/v) was used. The flow rate was 1.0ml/min and effluents were
monitored at 250nm. The retention time for Metoclopramide was 2.565 min. The method was validated
for linearity, accuracy, precision, specificity, limit of detection, limit of quantification and robustness.
Limit of detection and limit of quantification were found to be 0.03ppm and 0.099ppm respectively and
recovery of Metoclopramide from tablet formulation was found to be 8.73%. The proposed method was
successfully applied for the quantitative determination of Metoclopramide in tablet formulation.