A New Rp-Hplc Method Development and Validation for Simultaneous Estimation of Metoprolol Tartrate and Telmisartan in Pharmaceutical Tablet Dosage Form

The Present work was to develop a simple, fast, accurate, precise, reproducible, Reverse phase high performance liquid chromatographic method for simultaneous estimation of metoprolol tartrate and telmisartan in pharmaceutical tablet dosage form marketed as telsar beta. Chromatographic separation was done using Agilent – Zorbax SB-CN RP C18 column having dimension of 4.6×150mm having particle size of 3.5μm, with mobile phase consisting of phosphate buffer pH 3 ±0.02 pH adjusted with ortho phosphoric acid and methanol (30:70 %v/v), flow rate was adjusted to 0.8 ml/min and detection wavelength at 226nm. The retention times of metoprolol tartrate and telmisartan was found to be 2.3 and 4.2mins. The Proposed method has been validated for accuracy, precision, linearity, range and robustness were within the acceptance limit according to ICH guidelines. Linearity for metoprolol tartrate and telmisartan was found in range of 25μg-125μg & 20μg-100μg and correlation coefficient was found to be 0.999 and 0.999, %RSD for intermediate precision was found to be 0.11 and for repeatability was 0.40 and 0.23, % mean recovery for metoprolol tartrate and telmisartan was found to be 99.8% to 100.5%. The method was found to be robust even by change in the mobile phase ±5% and in less flow condition. The developed method can be successfully employed for the routine analysis of metoprolol tartrate and telmisartan in API and Pharmaceutical dosage forms.